|Product name||CREB-1 Monoclonal Antibody|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:1000), IHC-P (1:100-1:200).|
|Preparation method||The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen|
|Molecular weight||43 KD|
|Storage buffer||PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.|
|Storage instructions||Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||CREB1 (cAMP responsive element binding protein 1) encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of CREB1 results in several transcript variants encoding different isoforms.|
|Others||The antibody detects endogenous CREB-1 protein.|
Fig.1. Western blot analysis of 1) A431, 2) 3T3 using CREB-1 Monoclonal Antibody.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using CREB-1 Monoclonal Antibody.