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CK7 Monoclonal Antibody

CK7 Monoclonal Antibody

Views(318) Publications(0) Catalog no(ABM0018)
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Specification

Product name CK7 Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human, Mouse, Rat
Applications IF, IHC-P, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IF (1:50-1:200), IHC-P (1:200), IP (1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative KRT7; SCL; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 54 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background keratin 7 encoded by KRT7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Gene ID 3855
Alternative KRT7; SCL; Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Others The antibody detects endogenous CK7 proteins.
Accession P08729

Image & description

Fig.1. IHC-P staining of human lung cancer tissue, diluted at 1:200.

Fig.1. IHC-P staining of human lung cancer tissue, diluted at 1:200.

Fig.2. Western blot analysis of 1) Hela, 2) mouse kidney, 3) mouse brain, diluted at 1:2000.

Fig.2. Western blot analysis of 1) Hela, 2) mouse kidney, 3) mouse brain, diluted at 1:2000.

Fig.3. Immunofluorescence analysis of human liver tissue. 1, CK7 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunofluorescence analysis of human liver tissue. 1, CK7 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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