| Product name | CheKine™ Micro Total Iron-Binding Capacity (TIBC) Assay Kit |
| Applications notes | CheKine™ Micro Total Iron-Binding Capacity (TIBC ) Assay Kit provides a simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples. The principle is that under alkaline conditions, serum transferrin can be bonded to Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+, the bonded Fe3+ could be released after acidification ,and then be reduced into Fe2+ . A purple compound could be produced by the reaction between Fe2+ and philo oxazine ,and this purple compound has a special wave at 562 nm. Respectively reading the OD562nm values A1 and A2 , and A1 is based on the unbound Fe3+ , A2 is based on the bounded Fe3+ . And the concentration of TIBC is proportionate to the difference between A2 and A1. |
| Kit components | • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ-A • Reagent Ⅲ-B • Reagent Ⅳ • Standard |
| Features & Benefits | • simple, sensitive, rapid TIBC detection method, suitable for detecting serum samples . • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • If the absorbance values is too high (＞1.5). Pay attention to multiply by the dilution factor when calculating the result. • Please mix the standard well before use, it is normal while there is fewer precipitates in the standard solution. • Please take protective measures when operating, because ReagentⅡ has certain toxicity. |
| Storage instructions | The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Total Iron-Binding Capacity (TIBC ) refers to the ability of serum transferrin to combine iron, and its content is closely related to the occurrence of iron deficiency anemia, acute hepatitis and other diseases. |
Fig. Standard Curve of total iron-binding capacity (TIBC) Assay. The x-axis is total iron-binding capacity concentration (μmol/mL) and the y-axis is OD562.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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