| Product name | CheKine™ Micro Mitochondrial Transhydrogenase-1 (TH-1) Activity Assay Kit |
| SampleType | Animal and plant tissues |
| Alternative | Micro Mitochondrial Transhydrogenase-1 (TH-1) |
| Kit components | •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •Reagent Ⅴ |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Transhydrogenase (TH) is located in the inner membrane of mitochondria, also as known as Mitochondrial Respiratory Chain Complex VI. The enzyme catalyzes the mutual conversion of NADH+NADP + and NAD++NADPH, and regulate the balance between NAD(H) and NADP(H) in mitochondria. The forward reaction is called TH-1. The increase of mitochondrial NADH content will lead to the increase of H+ electrochemical gradient of mitochondrial membrane, thus promoting the production of ROS on the electron transport chain. TH-1 promotes the conversion of NADH to NADPH, thus improving the antioxidant capacity of mitochondria.CheKine TM Micro Mitochondrial Transhydrogenase-1 (TH-1) Activity Assay Kit can be used to detect biological samples such as animal and plant tissues. Both NADH and NADPH have characteristic absorption peak at 340 nm, therefore, the hydrogen transfer reaction catalyzed by TH cannot has a absorbance change at 340 nm. Replaces NADP + with 3-acetylpyridyl adenine dinucleotide (APADP + , synthetic substrate), TH-1 can catalyzes APADP + reduction to APADPH and the reaction product has a characteristic absorption peak at 375 nm. In this kit, the TH-1 activity is quantified by measuring the rate of increase in light absorption at 375 nm. |
| Alternative | Micro Mitochondrial Transhydrogenase-1 (TH-1) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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