| Product name | CheKine™ Micro Total Carbohydrate Assay Kit |
| Applications notes | CheKine™ Micro Total Carbohydrate Assay Kit provides a simple, convenient, rapid and colorimetric total carbohydrate detection method. The principle of kit is the total carbohydrates is hydrolyzed into reducing sugarsthe, and reducing sugars are heated with DNS reagents under alkaline conditions to reduce DNS to generate amino compounds, which are reddish-brown in alkaline solution and have a characteristic absorption peak at 540 nm. Within a certain concentration range, the reducing sugar content has a linear relationship with the absorbance at 540 nm. According to the standard curve, the total Carbohydrates content in the sample can be calculated. |
| Kit components | • Hydrochloric Acid • Sodium Hydroxide • DNS Reagent • Standard |
| Features & Benefits | • simple, sensitive, rapid Total Carbohydrate detection method. • compatible to animal or plant tissues, serum, plasma and other biological fluids. • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.If the sample value is higher than the maximum standard value, please further dilute the sample with distilled water. Pay attention to multiply by the dilution factor when calculating the result. • Hydrochloric Acid and Sodium Hydroxide has certain Corrosive. DNS Reagent has certain toxicity. Please take protective measures when operating. • This kit cannot achieve 100% degradation of cellulose. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Carbohydrate is one of the important components of animal and plant, and it is also the main raw material and storage substance of metabolism. Total carbohydrates mainly refer to reducing glucose, fructose, maltose, lactose, sucrose and maltose that can be hydrolyzed into reducing monosaccharides under the measuremen tconditions and starch that can possibly partially hydrolyzed . |
Fig.CheKine™ Micro Total Carbohydrate Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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