| Product name | Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST4 in samples. An antibody specific for MAST4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MAST4; KIAA0303; |
| Kit components | • Human Microtubule-associated serine/threonine-protein kinase 4 microplate • Human Microtubule-associated serine/threonine-protein kinase 4 standard • Human Microtubule-associated serine/threonine-protein kinase 4 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST4. No significant cross-reactivity or interference between Human MAST4 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MAST4 Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 5 isoforms of the human protein are produced by alternative splicing. Human protein kinases make up a large superfamily of homologous proteins, which are related by virtue of their kinase domains.MAST4 locates on human chromosome 5q13. The MAST4 cDNA is 7587 base pairs in length and encodes a putative protein of 2435 amino acids which contains a serine/threonine kinase domain and a PDZ domain. MAST4 protein has 64%, 63%, 59% and 39% identical aminoacid residues with MAST1, MAST2, MAST3 and MASTL respectively. RT-PCR analysis revealed relatively high expression level of MAST4 in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte. |
| Gene ID | 375449 |
| Alternative | MAST4; KIAA0303; |
| Accession | O15021 |
Fig.1. Human Microtubule-associated serine/threonine-protein kinase 4 (MAST4) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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