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CheKine™ Micro Plant Flavonoids Assay Kit

CheKine™ Micro Plant Flavonoids Assay Kit

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Product name CheKine™ Micro Plant Flavonoids Assay Kit
Applications notes The kit provides a simple method for detecting the flavonoid content in plant tissues. In the alkaline nitrite solution, the flavonoids and aluminum ions in the plant samples form a red complex with a characteristic absorption peak at 502 nm. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 502 nm.
Alternative bioflavonoids

Product Properties

Kit components • Nitrite Solution
• Chromogen
• NaOH Solution
• Quercetin Standard(10mg/mL)
Features & Benefits • Determination of flavonoid content in plant tissues.
• A broad range linearity: 0.156-10 mg/g.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Calibration range 0.156-10 mg/g
Limit of detection 0.078 mg/g
Usage notes • Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
• Each sample needs to set up a control well, the OD value is greater than 0.6, the sample is appropriately diluted and then measured, pay attention to the calculation formula multiplied by the dilution factor.
• Measure immediately after color development, and the absorbance will decrease after 2 hours.
Storage instructions Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Flavonoids are a class of polyphenyl compounds, which are secondary metabolites of plants. They have anti-inflammatory, antibacterial, hypolipidemic effects on the human body, scavenging hydroxyl free radicals in the body and preventing cancer. Quercetin is a typical flavonoid.
Alternative bioflavonoids

Image & description

Fig. Standard Curve of  flavonoid assay. The x-axis is OD502 and the y-axis is quercetin concentration (mg/mL) .

Fig. Standard Curve of flavonoid assay. The x-axis is OD502 and the y-axis is quercetin concentration (mg/mL) .

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