| Product name | CheKine™ Micro α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit |
| Applications notes | CheKine™ Micro α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit provides a simple, sensitive, rapid and colorimetric α-KGDH activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi. The principle of detection is that α-KGDH can catalyze α-Ketoglutarate,NAD + and coenzyme A to produce succinyl-coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. α-KGDH activity could be calculated according to NADH-generating rates. |
| Kit components | • Extraction Buffer • ReagentⅠ • Reagent Ⅱ • Reagent Ⅲ • Reagent Ⅳ • Reagent Ⅴ • Reagent Ⅵ • Reagent Ⅶ • Reagent Ⅷ |
| Features & Benefits | • simple, convenient, rapid and colorimetric α-KGDH activity detection method. • compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi . • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • ΔA of Test Tube should be between 0.01-0.3,the sample should be diluted further if ΔA of Test Tube ＞0.3. •Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. •Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • All samples and reagents should be on ice to avoid denaturation and deactivation . • Don't recommend to test too many samples in one time ,because enzyme activity is calculated by the variation of the absorbance value per unit time . •Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight , the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction. •Please to extract α-KGDH from fresh samples in order to ensure the enzyme activity . • Please reduce the tissues weight if the OD values is too high . Or reduce the volume of Extraction Buffer if the OD values is too low . |
| Storage instructions | The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | α-Ketoglutarate Dehydrogenase (α-KGDH) is widely present in mitochondria of the animal, plant, microorganism and in-vitro cultured cells. It is one of the key regulation enzyme of Kreb's Cycle, and can catalyze α-Ketoglutarate oxidation and decarboxylation to produce succinyl coenzyme A. |
Fig.CheKine™ Micro α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit (Colorimetric)
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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