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CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit

CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit

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Specification

Product name CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit
Applications notes CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit provides a convenient tool for detection of Hydroxyl Radical Antioxidant Capacity (HORAC). The principle is that sodium fluorescein as fluorescent probe, Fenton reaction produces hydroxyl radical, according to the damage of hydroxyl radical to fluorescent probe, the fluorescence intensity changes, the change of fluorescence intensity reflects the degree of free radical damage.

Product Properties

Kit components •Assay Buffer (2×)
•Reagent I (100×)
• Reagent II (32×)
• Reagent III
• Trolox Standard (5 mM)
Features & Benefits • Determination of Hydroxyl Radical Antioxidant Capacity (HORA) in a variety of biological samples such as serum, plasma, animal and plant tissues.
• Detailed sample preparation and result calculation methods are provided.
Calibration range 1.25-40 μM
Limit of detection 1.25 μM
Storage instructions Storage at-20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Hydroxyl Radical Absorbance Capacity (HORAC) is a method to detect the antioxidant Capacity of biological molecules in various samples, which can reflect the ability of antioxidants to transfer Hydroxyl free radicals.

Image & description

Fig. CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit

Fig. CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit

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