|Product name||CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit|
|Applications notes||CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit provides a convenient tool for detection of Hydroxyl Radical Antioxidant Capacity (HORAC). The principle is that sodium fluorescein as fluorescent probe, Fenton reaction produces hydroxyl radical, according to the damage of hydroxyl radical to fluorescent probe, the fluorescence intensity changes, the change of fluorescence intensity reflects the degree of free radical damage.|
|Kit components||•Assay Buffer (2×)
•Reagent I (100×)
• Reagent II (32×)
• Reagent III
• Trolox Standard (5 mM)
|Features & Benefits||• Determination of Hydroxyl Radical Antioxidant Capacity (HORA) in a variety of biological samples such as serum, plasma, animal and plant tissues.
• Detailed sample preparation and result calculation methods are provided.
|Calibration range||1.25-40 μM|
|Limit of detection||1.25 μM|
|Storage instructions||Storage at-20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Hydroxyl Radical Absorbance Capacity (HORAC) is a method to detect the antioxidant Capacity of biological molecules in various samples, which can reflect the ability of antioxidants to transfer Hydroxyl free radicals.|
Fig. CheKine™ Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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