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Human GM-CSF protein

Human GM-CSF protein

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Product name Human GM-CSF protein
Sequence Amino acid sequence derived from human GM-CSF isoform (P04141-1) (Ala18-Glu144) was expressed.
Activity Measured in a cell proliferation assay using TF-1 human erythroleukemia cells. The ED50 for this effect is 0.05-0.5 ng/mL.
Protein length The recombinant human GM-CSF consists of 128 amino acids and has a predicted molecular mass of 14 kDa.
Preparation method E. coli
Purity >98 % as determined by SDS-PAGE
Alternative GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Features & Benefits Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
Molecular weight 14 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GM-CSF protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human GM-CSF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.7% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).
Gene ID 1437
Alternative GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2
Accession P04141

Image & description

Fig. SDS-PAGE analysis of Human GM-CSF protein.

Fig. SDS-PAGE analysis of Human GM-CSF protein.

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