| Product name | Human GM-CSF protein |
| Sequence | Amino acid sequence derived from human GM-CSF isoform (P04141-1) (Ala18-Glu144) was expressed. |
| Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemia cells. The ED50 for this effect is 0.05-0.5 ng/mL. |
| Protein length | The recombinant human GM-CSF consists of 128 amino acids and has a predicted molecular mass of 14 kDa. |
| Preparation method | E. coli |
| Purity | >98 % as determined by SDS-PAGE |
| Alternative | GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2 |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits | Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 14 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GM-CSF protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human GM-CSF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.7% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML). |
| Gene ID | 1437 |
| Alternative | GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2 |
| Accession | P04141 |
Fig. SDS-PAGE analysis of Human GM-CSF protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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