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Cell Cycle Staining Kit

Cell Cycle Staining Kit

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Specification

Product name Cell Cycle Staining Kit
Applications notes Abbkine Cell Cycle Staining Kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content, providing a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Product Properties

Kit components • Nuclear Dye (50×)
• Assay Buffer (10×)
• RNase A (100×)
Features & Benefits • Flow cytometric analysis of cell cycle progression.
• Ready-to-use reagents for staining cells.
• The appropriate observe channel is Ex/Em=535/615 nm.
Usage notes The optimal concentration of the Nuclear Dye and incubation time varies depending on the specific application. The staining conditions may need modified according to the particular cell type.
Storage instructions Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The most common approach to determining the cell cycle stage (G1, S, and/or G2/M) is based on measurement of cellular DNA content. As cells progress through the cell cycle, cells in the G1 phase have one set of paired chromosomes and are uniform with respect to DNA content. On the other hand, the amount of DNA begins to double during S phase, so that the amount of DNA is between one and two times the amount in G1. Cells in G2/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes.

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