Login Register
English
0

Cart

$ 0

Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit

Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit

Views(603) Publications(0) Catalog no(KTE60646)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SKP2 in samples. An antibody specific for SKP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SKP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SKP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SKP2; FBL1; FBXL1; FLB1; MGC1366; CDK2/cyclin A-associated protein p45; S-phase kinase-associated protein 2

Product Properties

Kit components • Human S-phase kinase-associated protein 2 microplate
• Human S-phase kinase-associated protein 2 standard
• Human S-phase kinase-associated protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SKP2. No significant cross-reactivity or interference between Human SKP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background S-phase kinase-associated protein 2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase.
Gene ID 6502
Alternative SKP2; FBL1; FBXL1; FLB1; MGC1366; CDK2/cyclin A-associated protein p45; S-phase kinase-associated protein 2
Accession Q13309

Image & description

Fig.1. Human S-phase kinase-associated protein 2 (SKP2) Standard Curve.

Fig.1. Human S-phase kinase-associated protein 2 (SKP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human S-phase kinase-associated protein 2 (SKP2) ELISA Kit”