| Product name | CD4 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human CD4 around the non-phosphorylation site of S433 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:100-300;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 51kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CD4 encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. CD4 molecule functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in CD4. |
| Gene ID | 920 |
| Alternative | CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4 |
| Others | CD4 Polyclonal Antibody detects endogenous levels of CD4 protein. |
| Accession | P01730 |
| Observed Band(KD) | 51 |
Fig.1. Western Blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of COLO205 cells using CD4 Polyclonal Antibody diluted at 1:500.
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