|Product name||Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit|
|Applications notes||This Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF18 in samples. An antibody specific for TM4SF18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF18 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Transmembrane 4 L6 family member 18 microplate
• Human Transmembrane 4 L6 family member 18 standard
• Human Transmembrane 4 L6 family member 18 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit has high sensitivity and excellent specificity for detection of Human TM4SF18. No significant cross-reactivity or interference between Human TM4SF18 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||TM4SF18 also known as AAB5 and L6D, was cloned from human umbilical vein endothelial cells. It shares approximately 40 - 50% amino acid sequence identity with L6, IL-TMP, and TM4SF5, a group of proteins with limited homology to the tetraspanin superfamily. TM4SF18 belongs to the L6 tetraspanin family, L6, IL-TMP, and TM4SF5 are cell surface proteins predicted to have four transmembrane domains. Previous sequence analysis led to their assignment as members of the tetraspanin superfamily.A new sequence (L6D) that is strikingly similar to L6, IL-TMP, and TM4SF5. Analyses of these four sequences indicate that they are not significantly related to genuine tetraspanins, but instead constitute their own L6 superfamily.|
Fig.1. Human Transmembrane 4 L6 family member 18 (TM4SF18) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.