Product Information

• Product name

  CD2 Monoclonal Antibody

• Immunogen

  Synthetic Peptide

• Host

  Mouse

• Reactivity

  Human, Mouse, Rat

• Applications

  IHC-P

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:200).

• Clonality

  Monoclonal

• Isotype

  Mouse IgG1

• Purification

  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Fig.1. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, CD2 Monoclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue. 1, CD2 Monoclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded rat spleen tissue. 1, CD2 Monoclonal antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Product Properties

• Formulation

  Liquid solution

• Concentration

  1 mg/ml

• Storage buffer

  PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  CD2 molecule encoded by CD2 is a surface antigen found on all peripheral blood T-cells. CD2 molecule interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of CD2.

• Gene ID

  914

• Alternative names

  CD2; SRBC; T-cell surface antigen CD2; Erythrocyte receptor; LFA-2; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD2

• Others

  The antibody detects endogenous CD2 proteins.

• Accession

  P06729