Login Register
English
0

Cart

$ 0

Caspase-3 (phospho Ser150) Polyclonal Antibody

Caspase-3 (phospho Ser150) Polyclonal Antibody

Views(134) Publications(0) Catalog no(ABP56700)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Caspase-3 (phospho Ser150) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Caspase-3 around the phosphorylation site of S150
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.
Gene ID 836
Alternative CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Others Phospho-Caspase-3 (S150) Polyclonal Antibody detects endogenous levels of Caspase-3 protein only when phosphorylated at S150.
Accession P42574
Observed Band(KD) 34

Image & description

Fig. Western blot analysis of HBE using p-Caspase-3 (S150) antibody. Antibody was diluted at 1:1000.

Fig. Western blot analysis of HBE using p-Caspase-3 (S150) antibody. Antibody was diluted at 1:1000.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Caspase-3 (phospho Ser150) Polyclonal Antibody”