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CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody

CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody

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Specification

Product name CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody
Immunogen Synthesized peptide derived from human CaMKIIβ/γ/δ around the phosphorylation site of T287
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; Calcium/calmodulin-dependent protein kinase type II subunit gamma;

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 50, 65 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The product of CAMK2B (calcium/calmodulin dependent protein kinase II beta) belongs to the serine/threonine protein kinase family and to the Ca (2+) /calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of CAMK2B is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants.
Gene ID 816/818/817
Alternative CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; Calcium/calmodulin-dependent protein kinase type II subunit gamma;
Others Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody detects endogenous levels of CaMKIIβ/γ/δ protein only when phosphorylated at T287.
Accession Q13554/Q13555/Q13557

Image & description

Fig.1. Western Blot analysis of 22RV1 cells using Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of 22RV1 cells using Phospho-CaMKIIβ/γ/δ (T287) Polyclonal Antibody diluted at 1:500.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue. 1, CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue. 1, CaMKIIβ/γ/δ (phospho Thr287) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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