Product name | Apoptosis DNA Ladder Extraction Kit With Spin Column |
SampleType | Animal tissues, cells |
Alternative | DNA Ladder |
Kit components | •Lysis Buffer A •Lysis Buffer B •Wash Buffer A •Wash Buffer B •Elution Buffer •Spin Column •Collection Tube •RNase A •Proteinase K |
Features & Benefits | The verified samples are complete in type and easy to operate. |
Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
Storage instructions | Stored separately according to the label prompts of each component, the shelf life is 12 months Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Cellular endonucleases are activated and cut genomic DNA between nucleosomes into 180-200bp DNA fragments in cells undergoing apoptosis. When DNA is extracted for electrophoresis detection during cell apoptosis, DNA Ladder of 180-200 bp or its integral multiple can be found. DNA Ladder is a hallmark event in cells undergoing apoptosis, usually observed DNA ladder, cell apoptosis can be determined. Apoptotic DNA Ladder Extraction Kit With Spin Column uses DNA Spin Column to extract DNA Ladder, which is more convenient than traditional phenol chloroform extraction method for alcohol precipitation, and small DNA fragments are less likely to be lost. It is very efficient to extract small DNA Ladder down to180-200 bp in length, at the same time, genomic DNA up to 50 kb is also extracted. |
Alternative | DNA Ladder |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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