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ApoA-I Polyclonal Antibody

ApoA-I Polyclonal Antibody

Views(64) Publications(0) Catalog no(ABP53170)
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Specification

Product name ApoA-I Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human ApoA-I
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative APOA1; Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background APOA1 encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Gene ID 335
Alternative APOA1; Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1
Others ApoA-I Polyclonal Antibody detects endogenous levels of ApoA-I protein.
Accession P02647

Image & description

Fig.1. Western Blot analysis of K562 cells using ApoA-I Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of K562 cells using ApoA-I Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

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