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Anti-Trx Tag Mouse Monoclonal Antibody (14D4)

Anti-Trx Tag Mouse Monoclonal Antibody (14D4)

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Specification

Product name Anti-Trx Tag Mouse Monoclonal Antibody (14D4)
Immunogen Recombinant Protein
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative ADF; ATL derived factor; SASP; Surface associated sulphydryl protein; Thioredoxin; TRDX; TRX 1; Trx; TRX1; TXN; TXN protein

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Thioredoxin is a class of small redox proteins known to be present in all organisms. It plays a role in many important biological processes, including redox signaling. The thioredoxin (Trx) fusion E. coli expression system is available to offer soluble expression of normally insoluble or difficult to express proteins. It was reported that a number of mammalian cytokines and growth factors, when expressed as C-terminal trxA fusion proteins, stayed remarkably soluble in the E. coli cytoplasm under certain conditions.
Alternative ADF; ATL derived factor; SASP; Surface associated sulphydryl protein; Thioredoxin; TRDX; TRX 1; Trx; TRX1; TXN; TXN protein

Image & description

Fig. Western blot analysis of 1ug Trx Tag fusion protein with Anti-Trx Tag monoclonal antibody in 1:3000 (lane A) and 1:5000 (lane B) dilutions.

Fig. Western blot analysis of 1ug Trx Tag fusion protein with Anti-Trx Tag monoclonal antibody in 1:3000 (lane A) and 1:5000 (lane B) dilutions.

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