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Mouse Syntaxin-2 (STX2) ELISA Kit

Mouse Syntaxin-2 (STX2) ELISA Kit

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Specification

Product name Mouse Syntaxin-2 (STX2) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Syntaxin-2 (STX2) ELISA Kit employs a two-site sandwich ELISA to quantitate STX2 in samples. An antibody specific for STX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative STX2; EPIM; EPM; MGC51014; STX2A; STX2B; STX2C; epimorphin

Product Properties

Kit components • Mouse Syntaxin-2 microplate
• Mouse Syntaxin-2 standard
• Mouse Syntaxin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Syntaxin-2 (STX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse STX2. No significant cross-reactivity or interference between Mouse STX2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Syntaxins are a family of membrane integrated Q-SNARE proteins participating in exocytosis.Syntaxins possess a single C-terminal transmembrane domain, a SNARE domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxin-2 is a protein belongs to the syntaxin/epimorphin family of proteins. The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. The product of this gene regulates epithelial-mesenchymal interactions and epithelial cell morphogenesis and activation. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alternative STX2; EPIM; EPM; MGC51014; STX2A; STX2B; STX2C; epimorphin
Accession Q00262

Image & description

Fig.1. Mouse Syntaxin-2 (STX2) Standard Curve.

Fig.1. Mouse Syntaxin-2 (STX2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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