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Anti-RFP Tag Mouse Monoclonal Antibody (9D1)

Anti-RFP Tag Mouse Monoclonal Antibody (9D1)

Views(598) Publications(2) Catalog no(ABT2120)
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Specification

Product name Anti-RFP Tag Mouse Monoclonal Antibody (9D1)
Immunogen Recombinant Protein
Host Mouse
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative RFP; GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611

Product Properties

Formulation Liquid solution
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RFP (Red Fluorescent Protein), cloned from Discosoma coral (DsRed, DsRed2 or drFP583), is a protein which exhibits bright red fluorescence when exposed to blue light. It is common research practice for biologists to introduce a gene (or a gene chimera) encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
Alternative RFP; GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611

Image & description

Fig. Western blot analysis of 1ug RFP fusion protein with Anti-RFP monoclonal antibody in 1:2000 (lane A) , 1:3000 (lane B) and 1:5000 (lane C) dilutions.

Fig. Western blot analysis of 1ug RFP fusion protein with Anti-RFP monoclonal antibody in 1:2000 (lane A) , 1:3000 (lane B) and 1:5000 (lane C) dilutions.

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