| Product name | Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Reactivity | Mammals, Bacteria |
| Applications | IF, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:200). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | mCherry; mCherry tag |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry, derived from a protein isolated from Discosoma sp., is a 28.8kD monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores. |
| Alternative | mCherry; mCherry tag |
Fig.1. Immunofluorescence staining (1:200) of mCherry fusion protein in 293 cells with red.
Fig.2. Western blot analysis of 1ug mCherry fusion protein with Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) in 1:2000 (lane A) and 1:5000 (lane B) dilutions.
Author：Liu, Shiyan, et al. Publication name：Nature Communications IF：16.6
Author：Yalan Deng, Yuzhong Xiao, et al Publication name：Diabetes. 2018; 67(4): 569-580. IF：5.64
Author：Yuanyang Wang, Shuai Yan, et al Publication name：Diabetes. 2018; 67(9): 1748-1760. IF：5.64
Author：G Huang, Z Liu, B Gu, H Zhao, J Jia Publication name：Molecular plant pathology,2019. IF：4.36
Author：Zheng J, Chen Y, Li Z, et al Publication name：FASEB J, 2018, 32(2): 906-919. IF：4.32
Author：Guiyan Huang, Zhirou Liu, Biao Gu, et al Publication name： Mol Plant Pathol. 2019 Mar;20(3):356-371. IF：4.188
Author：Wu, D, et al Publication name：American Journal of Physiology-Endocrinology and Metabolism, 2016, 310(3): E225-E237. IF：3.89
Author：DD You, XL Zhou, QQ Wang, JL Liu Publication name：Experimental Cell Research IF：3.7
Author：Ma F, Wang L and Wang Y Planta, 2018 Publication name： IF：3.35
Author：Gao X, Xu F, Zhang H T, et al Publication name：Biochemistry and Cell Biology, 2016, 94(3): 256-264. IF：1.99
Author：Xiao G. Publication name：Research Square IF：/
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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