| Product name | Anti-DDDDK Tag Rabbit Polyclonal Antibody |
| Immunogen | Synthetic Peptide |
| Host | Rabbit |
| Reactivity | Mammals, Bacteria |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen |
| Alternative | DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
| Alternative | DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope |
Fig. Western blot analysis of 1ug Flag fusion protein with Anti-Flag Rabbit Polyclonal Antibody in 1:3000 (lane A) and 1:6000 (lane B) dilutions.
Author：Chen, Feng, et al. Publication name：Journal of Translational Medicine IF：7.4
Author：Xiao-Tao Zeng, Qi-Ya Zhang Publication name：Viruses. 2019 May 4;11(5). IF：4.03
Author：Linjiang Yang , Bingjie Shen , Mingshu Wang Publication name：Vet Microbiol IF：3.03
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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