| Product name | Anti-β-Actin Rabbit Polyclonal Antibody |
| Immunogen | Recombinant Protein |
| Host | Rabbit |
| Reactivity | Chicken, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus laevis |
| Applications | IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000), IHC-P (1:200). |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen |
| Alternative | ACTB; Actin, cytoplasmic 1; Beta-actin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACTB encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Gene ID | 60 |
| Alternative | ACTB; Actin, cytoplasmic 1; Beta-actin |
| Others | The antibody detects endogenous β-actin protein. |
| Accession | P60709 |
| Observed Band(KD) | 43 |
Fig.1. Western blot analysis of HepG2 (1), Rat liver (2), Mouse kidney (3), Rabbit testic (4), Sheep lung (5), 293T (6), diluted at 1:5000. Secondary antibody was diluted at 1:20000.
Fig.2. IHC-P staining of Human ovary tissue, diluted at 1:200.
Author：Sisi Cao, Zhuo Wang, et al Publication name：Journal of Experimental & Clinical Cancer Research. 2018 Sep 6;37(1):220. IF：5.34
Author：Han J, Lin M, Zhou D, et al Publication name：Cellular Physiology and Biochemistry, 2016, 38(5): 1803-1814. IF：4.81
Author：Lu H, Mei C, Yang L, Zheng J, Tong J, Duan F, Liang H, Hong L, Publication name：Front Pharmacol IF：4.225
Author：Y Xu, Y Jiang, L Wang, J Huang, J Wen, H Lv, X Wu Publication name：Neuroscience Bulletin, August 2019, Volume 35, Issue 4, pp 637–648. IF：3.31
Author：Yunlong Xu, Yanjun Jiang, Lin Wang, et al Publication name：Neurosci Bull. 2019 Feb 21. IF：3.155
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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