| Product name | Annexin I Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human Annexin I protein at amino acid sequence of 130-180 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | IF,WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 38kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein ANXA1 inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. ANXA1 (Annexin A1) is a Protein Coding gene. Diseases associated with ANXA1 include Hairy Cell Leukemia and Brain Edema. Among its related pathways are Prostaglandin Synthesis and Regulation and Signaling by GPCR. |
| Gene ID | 301 |
| Alternative | Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35 |
| Others | The antibody detects endogenous levels of Annexin I |
| Accession | P04083 |
Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.2. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Annexin I Polyclonal antibody was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.
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