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PCAF (Acetyl Lys428) Polyclonal Antibody

PCAF (Acetyl Lys428) Polyclonal Antibody

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Specification

Product name PCAF (Acetyl Lys428) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human PCAF around the acetylation site of K428
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. lysine acetyltransferase 2B associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that lysine acetyltransferase 2B plays a direct role in transcriptional regulation.
Gene ID 8850
Alternative KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF
Others Acetyl-PCAF (K428) Polyclonal AntibodySynthesized peptide derived from the human PCAF around the acetylation site of K428.
Accession Q92831
Observed Band(KD) 93

Image & description

Fig.1. Western Blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.1. Western Blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.2. Western Blot analysis of HepG2 cells using Acetyl-PCAF (K428) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

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