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Annexin I Polyclonal Antibody

Annexin I Polyclonal Antibody

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Specification

Product name Annexin I Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Annexin I protein at amino acid sequence of 130-180
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:200-1:500), ELISA (1:10000-1:20000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
Alternative Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 38 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein ANXA1 inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. ANXA1 (Annexin A1) is a Protein Coding gene. Diseases associated with ANXA1 include Hairy Cell Leukemia and Brain Edema. Among its related pathways are Prostaglandin Synthesis and Regulation and Signaling by GPCR.
Gene ID 301
Alternative Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Others The antibody detects endogenous levels of Annexin I
Accession P04083

Image & description

Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2,  Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2,  Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.3. Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Annexin I Polyclonal antibody was diluted at 1:200 (4°C,overnight). 2,  Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Annexin I Polyclonal antibody was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.

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