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α-tubulin (Acetyl Lys40) Monoclonal Antibody (5H5)

α-tubulin (Acetyl Lys40) Monoclonal Antibody (5H5)

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Specification

Product name α-tubulin (Acetyl Lys40) Monoclonal Antibody (5H5)
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. TUBA4A (tubulin alpha 4a) encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for TUBA4A.
Gene ID 7277
Accession P68363
Observed Band(KD) 52

Image & description

Fig.1. Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +) , using Acetyl- a-tubulin (Lys40) Mouse mAb 1:2000.

Fig.1. Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +) , using Acetyl- a-tubulin (Lys40) Mouse mAb 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.

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