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Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

Views(521) Publications(23) Catalog no(ABT2010)
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Specification

Product name Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)
Immunogen Synthetic Peptide
Host Mouse
Applications IF, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Alternative DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope

Image & description

Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.

Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.

Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).

Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).

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