Tips Shareing | Supertotal recombinant Protein Usage Guide
Recently, Abbkine has added a new batch of strictly verified active cytokine recombinant proteins to the shelves, many partners are consulting the use of recombinant protein precautions and preservation methods. Here it comes~ Abbkine recombinant proteins are freeze-dried powders that can be stored for several years at -20 °C or -80 °C. The lyophilized protein powder should be dissolved before use. Some recombinant protein products have a resolubilization buffer, and the matching resolubilization buffer is preferred. Resolubility and storage conditions of different recombinant proteins may be different. Refer to the recommended methods in the manual for details. Dissolution methods Always centrifuge the product before opening the tube. Centrifuge at 10,000-12000 rpm for 30 s or 3000-3500 rpm for 5…
New Products in September, Ready to Go
Abbkine has been adhering to the spirit of innovation, constantly pioneering and striving to study and practice, as expected, a wave of new products, involving a wide range of experimental applications, mainly divided into cell lines, cytokines, biochemical detection kits, double antibody, mycoplasma PCR detection kits, tool enzymes and other series. Come Kangkang have their own needs ~ Cytokines (Low endotoxin & High Purity & Freeze-dried powder) Cytokine is a kind of unique cell signal transduction molecule, which plays an important role in cell proliferation and development. Abbkine provides cytokines including interleukin, chemokines, colony-stimulating factor (CSF), interferon and the transforming growth factor (TGF) and tumor necrosis factor (TNF) families for use in cancer, apoptosis, endocrinology, immunology, neuroscience, protease and stem…
Such excellent special fetal bovine serum, how can anyone not know?
When the delicate cells need for laboratory to develop, you need a high quality premium fetal bovine serum; When cell toxicology experiments need to operate under GMP conditions, you need a high-quality special grade fetal bovine serum; When precious cell cultures need to avoid contamination of the serum source, you need a premium fetal calf serum; When there is a lack of quality assurance in cell culture serum, you need a good quality extra grade fetal bovine serum. Bovine serum is the largest natural medium used in cell culture, containing rich nutrients necessary for cell growth, and is often used in cell culture in vitro.The pH value, osmotic pressure, hemoglobin concentration, endotoxin level, total protein and other indicators of…
A scientific mystery lit up by fireflies -- dual luciferase reporter gene
Do you want to verify the effect of miRNA on target genes? -- Dual luciferase reporter gene assay Do you want to understand the targeted interaction between miRNA and lncRNA/circRNA? -- Dual luciferase reporter gene assay Do you want to know what the transcription factor is to a downstream gene? -- Dual luciferase reporter gene assay Yes, the dual luciferase system is so powerful! Dual luciferase reporter genes usually use firefly luciferase as the reporter gene and renilla luciferase as the reference gene. The reporter system has the advantages of rapid detection, high sensitivity, wide dynamic range and no intracellular active interference. It is widely used in the research fields of gene regulation and non-coding RNA targeted interaction. Today, we will…
Summer stock season, biochemical kits have been equipped for you
Biochemical kits are obviously used for biochemical testing, but what is biochemical testing? Biochemical detection, as the name suggests, refers to the use of biological or chemical methods to detect samples. The determination indexes are usually biochemical indexes such as enzymes, sugars, lipids, protein and non-protein nitrogen, inorganic elements, etc., or body function indexes. Common biochemical tests can be divided into two simple categories: enzyme activity tests and substrate or product tests. For example, according to the specific effects of the indicators, it can be divided into: oxidation and antioxidant series, glutathione series, amino acid metabolism series, lipid metabolism series, nitrogen metabolism series, coenzyme I series, coenzyme II series, neurotransmitter series, vitamin series, sugar metabolism series, ion series, esterase series,…
CheKine™|Operation difficulties summer customs clearance
Hello, everyone. Recently, the Abbkine CheKine™ cell metabolomics product line has been popular again! The reason for the fire is very simple: they are beautifully packaged, superior materials, excellent experimental results, and open the door to the quantitative detection of biochemical substances. Abbkine CheKine™ cell metabolomics product line includes a large number of related metabolites, enzymes, nutrition and metabolite quantitative detection kits, etc. Abbkine CheKine™ cell metabolomics product line includes a large number of related metabolites, enzymes, nutrition and metabolite quantitative detection kits, etc. Features and Advantages High sensitivity, the method is based on optimized colorimetry, higher sensitivity, no radioactivity, safe and stable; Suitable for a variety of samples, including animal and plant tissues, cells, bacteria, fungi, serum, plasma; The experimental…
Big news! Here comes Nature article cites Abbkine antibody
Recently, the Nature online published the title "Genome editing of a rice CDP-DAG synthase confers multipathogen" completed by Professor Li Guotian's team at Huazhong Agricultural University (Wuhan, China) and several units. The team cloned a broad-spectrum disease-resistant spot mutant gene RBL1, and created a new gene RBL1δ12 through gene editing to enhance broad-spectrum disease resistance and stable yield of crops. The gene is highly conformed in crops, and compared with traditional disease resistance genes, it can break species boundaries and has greater universality, and has great application potential for disease resistance breeding. The research results will improve the resistance of diseases such as "rice cancer" by three levels, and the new gene can reduce the loss of rice yield by…
This summer without regret, SuperKine™ Lipo3.0 efficient transfection reagents rooting for the college entrance examination
In the process of passing cell transfection, friends may meet with difficulties, such as: The transfection efficiency is low, the signal is not obvious; The compatibility of low transfection reagent, vulnerable to the influence of serum and antibiotics; The transfection of siRNA regulation of gene expression effect is not obvious. Fig. The transfection effect of HEK293T cells (A), Hela cells (B), and Cos-7 cells (C) using Lipo3.0 Transfection Reagent The SuperKine ™ Lipo3.0 efficient transfection reagent is a very effective new type cationic liposome transfection reagent, applicable to a variety of animal cell plasmid DNA and siRNA transfection, for the majority of animal cell has a high transfection efficiency. Fig1 Transfection reagent transfection principle. With more advantages: High transfection efficiency:…
Immunohistochemical project | solve all immunohistochemical problems here
Congratulations! Click on this article, and you're halfway to your immunohistochemical experiment! Let's move on to the subject of today's discussion: immunohistochemistry Common Staining Methods for Immunohistochemistry Hematoxylin-Eosin (HE) Staining Hematoxylin-Eosin staining (HE) was one of the common staining methods for paraffin sectioning techniques. The main staining principle is that hematoxylin dyeing solution is alkaline, which can make the chromatin in the nucleus and nucleic acid in the cytoplasm appear blue. Eosin is an acidic dye that causes components in the cytoplasm and extracellular matrix to appear red. HE staining is the most basic and widely used technique in the teaching and research of histology, embryology and pathology. Diaminobenzidine (DAB) Staining Different from HE staining, DAB staining can detect protein…
Cell State detection project | cellular proliferation and apoptosis assay
Different cellular states Living cells: complete cell structure, stable metabolism, normal vitality; Senescent cells: cell cycle arrest; Toxic cells: cell proliferation activity decreased, metabolism slowed down; Apoptotic cells: cell lipid membrane eversion, membrane potential disappeared, DNA fragmentation, programmed death; Necrotic cell: Loss of membrane integrity, gradual dissolution of the cell membrane, release of contents. Cell proliferation assay Detection of cell proliferation is essential to assess cell health, determine genotoxicity or evaluate anticancer drugs, and direct measurement of DNA synthesis is currently the most accurate method. Compared with the BrdU method, EdU method does not require antibodies, is convenient to operate, and has high detection sensitivity. Based on the EdU incorporation and subsequent Click reaction, the covalent reaction between azide and alkynes catalyzed…
