Big news! Abbkine antibody cited in the cover article of Cell
Title: Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Key information preview Complex diseases often involve the interplay between genetic and environmental factors. This study reveals that abnormalities in membrane-free organelles are a key mechanism leading to peripheral neuropathy. Original link https://doi.org/10.1016/j.cell.2022.12.046 Abbkine products are cited A series of high quality documents witness Abbkine's consistent product quality! Abbkine IP series products, a large number of reliable literature references, boost your IP experiment! Recommended Products Cat No. Product name Size KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20 T KTD105-EN Universal IP/Co-IP Toolkit (Agarose) 20 T KTI1010-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) 20 T KTI1020-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit) 20 T A25022 IPKine™ HRP, Mouse Anti-Rabbit…
How to dectet Mitochondrial Permeability Transition Pore ?
Background Mitochondrial membrane permeability transition pores (MPTP) are nonspecific channels located in the inner and outer membranes of mitochondria that appear to be involved in the release of mitochondrial components during cell death. MPTP switching significantly changes mitochondrial permeability and mitochondrial membrane potential. This sustained pore activation is caused by mitochondrial Ca2+ overload, mitochondrial glutathione oxidation, elevated mitochondrial reactive oxygen species levels, and other pro-apoptotic conditions. MPTP is closely related to cell survival and apoptosis, and the detection of MPTP is also permeated in many fields, such as ischemia/reperfusion, tumor, aging, neurodegeneration, etc. We developed an imaging microanalysis and flow cytometry kit for detecting mitochondrial transition pore opening. We developed an both can conduct imaging microanalysis and flow cytometry kit…
Difficulties are broken one by one, and anyone who doesn't have such a good reagent kit will be heartbroken, okay？
I met a fellow student who was doing drug toxicity research, Cell drug exposure does a lot of work upfront, But one day The teacher came to him and said: You can go a little higher than normal, Delve into organelles and genetic material levels. The student was instantly depressed: With limited funds at hand, The conventional electron microscope equipment for organelle study was not available, Genetic material has never been studied at the molecular level before, So what? Are there a few conventional equipment, easy to operate experimental tools can solve the urgent needs of students? Abbkine Mitochondrial Permeability Transition Pore Assay, the opening of mitochondrial permeability transition pore is a key event that causes cell death and plays…
Good news! The latest high score cited literature of Abbkine products is coming out! Impact factor up to 49.29!
Abbkine IPkine light chain specific secondary antibody (Cat #: A25012; Cat #: A25022) was recently cited in Cancer Cell, a well-known journal in Medical Region. Title: Epithelial cells activate fibroblasts to promote esophageal cancer development First Author Affiliation: Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China Previews of abstracts Original link https://doi.org/10.1016/j.ccell.2023.03.001 Abbkine products are cited A series of high quality documents witness Abbkine's consistent product quality! Abbkine IP series products, a large number of reliable literature references, boost your IP experiment! Recommended Products Cat No. Product name Size KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20 T KTD105-EN Universal IP/Co-IP…
Summary and breakthrough of problems in immunoprecipitation experiment
In the cellular world, no protein is an island; each needs to interact with other proteins to function. The most common method of finding protein friends is immunoprecipitation (IP)/ co-immunoprecipitation (Co-IP). However, in the actual operation process of immunoprecipitation, Baozi will encounter many difficulties. Here, Xiaobian, with the spirit of hard work, panning for gold in the sand, selects the following frequently asked difficulties and makes a breakthrough to share with you. 1.What is the difference between co-immunoprecipitation and immunoprecipitation? The principles and methods used in co-immunoprecipitation and immunoprecipitation are roughly similar, except that in co-immunoprecipitation, the binding and precipitation of the target protein is replaced by another protein that interacts with it. On the basis of co-immunoprecipitation or…
IF project | Solve the difficult problems of IF one by one
Immunofluorescence (IF) is based on the principle of antigen-antibody reaction. Firstly, a known antigen or antibody is labeled with fluorescein to make a fluorescent antibody, and then the fluorescent antibody (or antigen) is used as a probe to detect tissue or cells. In the daily IF experiment, we spent all our energy and effort to get the samples, operated with full confidence and expected the final results, but sometimes we couldn't see any fluorescence signal or had ultra-high background signal, which made our hearts cool all at once. Even if you forget to eat and sleep, optimize the conditions, repeat the experiment, make a headache, still can't find the cause of the problem, began to fall into self-doubt, feeling…
Efficient & Sensitive EliKine™ ELISA kit, On Big Sale!
With years of experience in antibody development, after strict quality control test, Abbkine has launched EliKine™ ELISA Kits with high specificity and sensitivity. The detection target can be classified into cytokines, hormones and other proteins. EliKine™ ELISA Kits are validated with various samples collected from human, mouse and rat which can meet your diverse research needs. Part one：Features & Advantages of EliKine™ ELISA EliKine™ sandwich ELISA kits depend on paired capture and detection antibodies, which are both specific to the antigen with 2 epitopes, while competitive ELISA kits employ the competitive inhibition enzyme immunoassay technique with featured and specific detection antibodies. Exclusive EliKine™ Streptavidin-HRP conjugate, and HRP substrate with other optimal components make EliKine™ portfolio be one of the best…
Analysis and detection of antioxidant capacity
1、What is antioxidant capacity? There are many antioxidants in living organisms, such as SOD, CAT, GSHPx, G6PD, GR, glutathione, etc., which can remove various reactive oxygen species in the body to prevent the generation of oxidative stress induced by reactive oxygen species. The total level of various antioxidant macromolecules, antioxidant small molecules and enzymes in a system reflects the antioxidant capacity of the system. Although the analysis of ROS induced oxidative damage is widely used as an analysis indicator, it is also important to detect the level of antioxidant capacity of cells in vivo, cells and extracts. The antioxidant capacity of intracellular macromolecules can be measured by ORAC (antioxidant capacity of oxygen group), HORAC (antioxidant capacity of hydroxyl group) and…
How much we know about cell death
Programmed cell death is an active, orderly and gene-controlled process of cell death, including apoptosis, pyrodeath, autophagy and iron death. Apoptosis is the first kind of programmed cell death mode discovered by people, and it is also the most typical and the most clearly studied programmed cell death mode. Apoptosis is the normal physiological response of cells after receiving some specific signal stimulation. Apoptosis can be divided into two main categories according to its inducible factors: endogenous apoptosis (mitochondrial apoptosis pathway) and exogenous apoptosis (death receptor pathway). Endogenous apoptosis is mainly controlled by the BCL2 protein family, which includes pro-apoptotic factors BAX, BAK and BOK, and anti-apoptotic factors BCL2, BCLXL, BCLw and MCL1, etc. Through the interaction between pro-apoptotic factors…
Special Topics on column protein extraction| Abbkine ExKine™ Pro Series Kits
Disadvantages of conventional RIPA lysis buffer protein extraction The conventional RIPA lysis buffer is easy to produce insoluble components for protein extraction, resulting in random loss of proteins, incomplete protein obtained, affected protein activity, and low protein purity, thus limiting the applicability of protein samples obtained for downstream experiments. Figure 1. Abbkine Protein Extraction Kit (Column method) Figure 2. Comparison of solubility of Abbkine protein extraction kit (column method) lysates and conventional RIPA lysis buffer. (Sample: 293 cells) Compared with the conventional RIPA lysis buffer liquid phase of various strengths, the lysis buffer of Abbkine protein extraction kit (column method) showed higher protein solubility, and no soluble components were produced, which could solve the problem of total protein loss in solution…
