Cell Metabolism | Subfamily Inhibitor Product Reveals Liver Acetyl-CoA Metabolism Mechanism! Provides New Strategy for Treatment of Neuroinflammation and Susceptibility to Depression
Research Title: Hepatic Acetyl-CoA Metabolism Regulates Neuroinflammation and Depression Susceptibility Viaacetate Journal: Cell Metabolism Impact Factor: 30.9 Publication Date: 2025 Author Team: Chen Jianguo's Team at Huazhong University of Science and Technology Collaborative Product: KTA8010-Dual Luciferase Reporter Gene Detection Kit Original link: https://pubmed.ncbi.nlm.nih.gov/40992374 I. Research Background Previous studies have predominantly focused on "abnormal brain energy metabolism and depression," yet the role of peripheral organs—particularly the metabolic hub, the liver—in regulating mood remains an unresolved mystery. In clinical practice, patients with abnormal liver function are often accompanied by neuropsychiatric symptoms, suggesting that the "liver-brain axis" may be involved in mood regulation, but the specific molecular mechanism is unknown. Acetyl-CoA is a key metabolic intermediate, and the ACOT12 enzyme in the liver…
A decade of research on glycolysis: from energy factory to core of life regulation
The past decade has witnessed explosive breakthroughs in glycolysis research. Systematic progress has been made in subcellular localization, regulatory mechanisms, physiological and pathological functions, and clinical translation. These advancements not only reveal the complex regulatory network of this ancient metabolic pathway but also provide novel therapeutic targets and strategies for disease treatment and regenerative medicine. Below is a curated summary of the latest research highlights: dimension traditional view Breakthroughs in the last decade 1. Location and organizational structure The glycolysis is a soluble pathway which is evenly distributed in the cytoplasm. Precise subcellular partitioning regulation forms efficient local energy supply system 2. Core regulatory mechanisms Glycolysis is a passive, substrate-driven metabolic pathway, which is mainly regulated by classical feedback mechanisms…
Nanchang University won the 14 points with KTB1300 -CheKine™ Micro Glucose Assay Kit and KTA4001-Mitochondrial Membrane Potential Assay Kit (JC-1), revealing the scientific research accelerator behind it.
Journal：Advanced Science IF:14.1 Application products: Research content Epidemiological studies show that some diabetic patients develop end-stage renal dysfunction without significant proteinuria or glomerulopathy, underscoring the role of renal tubular epithelial cell (TEC) impairment in diabetic kidney disease (DKD). However, the primary pathogenic determinants underlying TEC impairment and disease advancement in DKD progression remain unclear. This study reveals that asprosin (ASP) is up-regulated and positively correlated with kidney dysfunction in DKD mice. Moreover, elevated ASP is mainly located in the renal TEC, and negatively impacts TEC. In addition, supraphysiological ASP concentration impairs mitochondrial dynamics and function in both DKD mice and HK2 cells. Mechanistically, ASP promotes Drp1 over-SUMOylation, thus reducing Drp1 degradation and disrupting mitochondrial dynamics homeostasis. However, the mutation of…
Partnership with Bio-Connect to Boost Development of Life Science and Diagnostic Reagents
We are pleased to announce our partnership with Bio-Connect, a leading distributor of life science and diagnostic reagents in the Netherlands, Belgium, Luxembourg and throughout Europe. With an extensive portfolio and a team of dedicated experts, Bio- Connect ensures that researchers have access to the high quality products they need. Their strong reputation as a trusted supplier in both the academic and industrial sectors makes this collaboration even more meaningful for us. Together, we look forward to providing customers in the Benelux region with innovative solutions and excellent support. To learn more about Bio-Connect and our partnership, please visit www.bio-connect.nl.
Cytokine References
Keywords: M1/M2 polarization; IL-4; IFN-γ; BMDM Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization Product Cited: PRP2117 -Mouse IL-4 protein PRP1015-Mouse IFN-γ protein, His Tag Affiliation Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China Application IFN-γ (50 ng/mL) and IL-4 (25 mg/mL) were added in the culture media for M1/M2 macrophage polarization. Result Nrf2 deficiency promotes M1 macrophage polarization and inhibits M2 macrophage polarization through autophagy modulation. (A,B) Flow cytometry analyses of M1 macrophage polarization in BMDMs. (C,D) Immunofluorescence staining of CD206 and F4/80 in BMDMs. White arrows in the pictures indicate polarized macrophages. (E) Isolated BMDMs were treated with LPS/IFN-γ (15 ng/mL and 50 ng/mL, respectively) for 24 h to induce M1 macrophage…
Frontiers of organoid technology: Revolutionary breakthroughs from disease models to Personalized medicine
Organoids are an advanced three-dimensional cell culture system that mimics the structure and function of real biological organs. The technology, which mainly relies on stem cells and tissue engineering, enables cells to self-assemble under appropriate culture conditions to form miniature organ-like structures with certain tissue properties and functions. The construction of organoids usually begins with stem cells, including adult stem cells, embryonic stem cells or induced pluripotent stem cells (iPSCs). With the help of specific growth factors and media, these stem cells can differentiate and self-organize into complex structures, similar to the many cell types and tissue layers found in native organs. Because of their ability to reproduce some of the physiological functions of organs outside the body, organoids have become important tools for biomedical research. Here are a few of the main areas of application for organoids: Diseasemodeling: Organoids provide a powerful platform for studying the onset and progression of disease. They can be used to mimic a variety of disease states, including hereditary diseases, tumors, infectious diseases, etc., thus helping scientists better understand the biological basis of disease. Drugdiscovery and testing : Since organoids can more truly reflect the physiological and pathological environment of human tissues, they are used in the initial screening and safety testing of drugs, helping to reduce the dependence on animal models and accelerate the drug development process. Personalizedmedicine : Organoids built using a patient's own stem cells can be used to predict an individual's response to a specific drug, guide precision medicine decisions, and achieve more effective personalized treatment. Regenerativemedicine : Organoid technology offers new avenues for tissue and organ repair, for example, skin organoids can be used to repair the skin of burn patients, while islet organoids may be a new hope for diabetes treatment. Toxicologicalresearch : Organoids can be used to assess the toxicity of chemicals, drugs or environmental factors to inform the development of safety standards and policies. Developmentalbiology : Organoids provide a unique insight into embryonic development and tissue formation, helping scientists to understand the mechanisms of cellular interactions during normal development. Gene-editingresearch : In combination with gene-editing techniques (such as CRISPR-Cas9), organoids are being used to explore the effects of genetic mutations on tissue function and promote greater understanding of inherited diseases. Multi-omicsand phylogenetic studies : Organoids provide experimental models for integrating genomic, transcriptomic, proteomic, and metabolomic data to help scientists interpret disease and treatment responses from a systems biology perspective. Celltherapy : Organoids can be used as a source of cells for cell therapy, such as transplanting specific types of cells into a patient to repair or replace damaged tissues or organs. Educationand training : Organoids are also used in medical education to help students and doctors intuitively understand the structure…
Such a complete cellular workup is now very rare
Abbkine provides you the most preferred products for cell research users, covering cell proliferation, cytotoxicity, apoptosis, cell aging, oxidative damage and other aspects of detection applications. The product types include innovative concepts, cell apoptosis and cell proliferation explosive kits with unique advantages, as well as superior quality and stable performance detection kits. To meet your different experimental needs and help your cytological research career. Detection of cell proliferation Cell proliferation produces many important changes, including DNA synthesis, increased cell metabolism, and expression of proliferation-specific proteins. Based on the next-generation AbFluor ™ fluorescent staining technology, Abbkine has developed products that can not only accurately detect cell proliferation directly from the DNA level, but also achieve high-throughput detection of cell proliferation status…
The coolest new products in the research circle are here! CheKine™ microquantitative test kits and recombinant proteins help you explore cells with ease!
Hey, research explorers! Just like that, the long-awaited tech products are dazzlingly here again! The latest CheKine™ product line helps you play freely in the field of microquantitative detection, with super cool recombinant proteins adding more excitement to cell exploration! Come and check out these trendy new favorites! Microquantitative test kits, exploring cellular energy Glycogen synthase activity assay kit: Not only fast and accurate, but also gives you a novel way to analyze cellular energy metabolism, turning you into a cellular energy superhero in seconds! Starch assay kit: Detailed and accurate, helping you deeply analyze the role of starch in organisms, playing with starch research, making you the little prince/princess of the starch research world! α-Glucosidase activity assay kit: Microquantitative…
To track the hot spots of mitochondria and solve the difficult problems in respiratory chain research
In addition to being a key organelle for intracellular energy generation, mitochondria are also involved in metabolic processes such as apoptosis, free radical production, and lipid metabolism. Some studies have reported that mitochondrial dysfunction contributes to the pathology of many common diseases, including neurodegeneration, metabolic diseases, heart failure, ischemia-reperfusion injury, and protozoan infections. Therefore, mitochondria represent an important drug target for these highly prevalent diseases. Several strategies aimed at therapeutic restoration of mitochondrial function are emerging, and a few agents have entered clinical trials. The role of the respiratory chain represents the most basic function of mitochondria. The hydrogen carrier and electron carrier in the respiratory chain are carriers that can transfer hydrogen atoms or electrons. Since hydrogen atoms can…
Tips Shareing | Supertotal recombinant Protein Usage Guide
Recently, Abbkine has added a new batch of strictly verified active cytokine recombinant proteins to the shelves, many partners are consulting the use of recombinant protein precautions and preservation methods. Here it comes~ Abbkine recombinant proteins are freeze-dried powders that can be stored for several years at -20 °C or -80 °C. The lyophilized protein powder should be dissolved before use. Some recombinant protein products have a resolubilization buffer, and the matching resolubilization buffer is preferred. Resolubility and storage conditions of different recombinant proteins may be different. Refer to the recommended methods in the manual for details. Dissolution methods Always centrifuge the product before opening the tube. Centrifuge at 10,000-12000 rpm for 30 s or 3000-3500 rpm for 5…
