| Product name | YAP (phospho Ser127) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human YAP around the phosphorylation site of S127 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | IF,WB,IHC,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications; |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 65kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | YAP1 encodes a downstream nuclear effector (Yes associated protein 1) of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. YAP1 is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Gene ID | 10413 |
| Alternative | YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65 |
| Others | Phospho-YAP (S127) Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at S127. |
| Accession | P46937 |
| Observed Band(KD) | 65 |
Fig.1. Western Blot analysis of MCF-7(1), PC-3(2), KB(3), Hela(4), diluted at 1:500.
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