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VPS72 Polyclonal Antibody

VPS72 Polyclonal Antibody

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Specification

Product name VPS72 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human VPS72 at AA range: 100-180
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative VPS72; TCFL1; YL1; Vacuolar protein sorting-associated protein 72 homolog; Protein YL-1; Transcription factor-like 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by VPS72 (vacuolar protein sorting 72 homolog) is a shared subunit of two multi-component complexes, the histone acetyltransferase complex TRRAP/TIP60 as well as the chromatin remodeling SRCAP-containing complex. The TRRAP/TIP60 complex acetylates nucleosomal histones important for transcriptional regulation, double strand DNA break repair and apoptosis. The SRCAP-containing complex catalyzes the exchange of histone H2A with the histone variant Htz1 (H2AFZ) into nucleosomes. This protein may be responsible for binding H2AFZ, which has a role in chromosome segregation. This protein may also have a role in regulating long-term hematopoietic stem cell activity. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Gene ID 6944
Alternative VPS72; TCFL1; YL1; Vacuolar protein sorting-associated protein 72 homolog; Protein YL-1; Transcription factor-like 1
Others VPS72 Polyclonal Antibody detects endogenous levels of VPS72 protein.
Accession Q15906
Observed Band(KD) 41

Image & description

Fig. Western blot analysis of 293T lysis using VPS72 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of 293T lysis using VPS72 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.

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