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Vimentin (phospho Ser83) Polyclonal Antibody

Vimentin (phospho Ser83) Polyclonal Antibody

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Specification

Product name Vimentin (phospho Ser83) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Vimentin around the phosphorylation site of S83
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative VIM; Vimentin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background VIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.
Gene ID 7431
Alternative VIM; Vimentin
Others Phospho-Vimentin (S83) Polyclonal Antibody detects endogenous levels of Vimentin protein only when phosphorylated at S83.
Accession P08670
Observed Band(KD) 54

Image & description

Fig. Western Blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody.

Fig. Western Blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody.

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