| Product name | Villin Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from Villin at AA range: 601-650 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | villin 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 90kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | VIL1 (villin 1) encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. |
| Gene ID | 7429 |
| Alternative | villin 1 |
| Others | Villin Polyclonal Antibody detects endogenous levels of Villin. |
| Accession | P09327 |
| Observed Band(KD) | 90 |
Fig.1. Western Blot analysis of K562 cells using Villin Polyclonal Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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