| Product name | VGF Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human VGF |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | VGF; Neurosecretory protein VGF |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 67kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. |
| Gene ID | 7425 |
| Alternative | VGF; Neurosecretory protein VGF |
| Others | VGF Polyclonal Antibody detects endogenous levels of VGF protein. |
| Accession | O15240 |
| Observed Band(KD) | 67 |
Fig.1. Western Blot analysis of 293 cells using VGF Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.
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