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VEGF-A Polyclonal Antibody

VEGF-A Polyclonal Antibody

Views(453) Publications(0) Catalog no(ABP52839)
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Specification

Product name VEGF-A Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human VEGF-A
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background VEGFA is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Gene ID 7422
Alternative VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
Others VEGF-A Polyclonal Antibody detects endogenous levels of VEGF-A protein.
Accession P15692
Observed Band(KD) 27

Image & description

Fig.1. Western Blot analysis of 22RV-1,  SW480, BT474, K562,  Mouse liver cells using VEGF-A Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of 22RV-1, SW480, BT474, K562, Mouse liver cells using VEGF-A Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:100.

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