| Product name | Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) |
| Applications notes | Based on the pain points of the traditional IP/Co-IP experiment, Abbkine developed a Universal IP/Co-IP Toolkit (Magnetic Beads); After designed and researched, the toolkit contains: optimized natural and denatured lysate, protein A/G magnetic beads, IP negative control, IPkineTM second antibody, it can meet the IP/Co-IP needs of most users |
| Kit components | • Non-Denaturing Lysis Buffer-25 mL • 10×Wash Buffer-20 mL • Protein A/G Magnetic Beads-0.5 mL • Elution Buffer-2 mL • Neutralization Buffer-0.2 mL • 100×Proteinase Inhibitor Cocktail-0.2 mL • Mouse IgG (1 mg/mL)-30 μL • IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL |
| Features & Benefits | • Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption. • Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time. • Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference. |
| Storage instructions | Store according to the recommended storage conditions of each component. |
| Shipping | Blue ice |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | Immunoprecipitation (Immunoprecipitation, IP) is a small affinity purification method using specific antibodies fixed on solid-phase supports such as magnetic beads or agarose. As an important part of many proteomics-related studies, IP can be used to detect the existence of proteins, relative abundance, up-and-down expression of proteins, stability and interaction of proteins and so on. |
Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.
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