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Ulp1 (SUMO Protease/Ulp1 peptidase)

Product Information

  • Product name

    Ulp1 (SUMO Protease/Ulp1 peptidase)

  • Sequence

    Amino acid sequence derived from ULP1 isoform (Q02724) (403-621 aa + N-terminal Poly-6*His tag C-terminal Poly-6*His tag) was expressed.

  • Protein length

    The recombinant yeast ULP1 consists of 219 amino acids and has a predicted molecular mass of 28.7 KD. The apparent molecular mass of the ULP1 is approximately 28 KD in SDS-PAGE under reducing conditions due to glycosylation.

  • Preparation method

    E. coli

  • Purity

    >90% as determined by SDS-PAGE.

Product Properties

  • Formulation

    Lyophilized from sterile 20 mM Tris , 500 mM NaCl pH8.0.

  • Molecular weight

    28.7 KD

  • Storage instructions

    Lyophilized ULP1 protein product should be stored desiccated below -80°C. Avoid freezing and thawing.

  • Shipping

    The product is shipped with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility.

  • Gene ID

    856087

  • Alternative names

    Ulp1 peptidase; SUMO Protease

  • Accession

    Q02724

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Ulp1 (SUMO Protease/Ulp1 peptidase)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: PRP3001

  • Size
  • Price
  • 500 U $80
  • 1000 U $140
  • 5000 U $630

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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