| Product name | UBE4A Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human UBE4A |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | UBE4A; KIAA0126; Ubiquitin conjugation factor E4 A |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 122kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | UBE4A encodes a member of the U-box ubiquitin ligase family. The ubiquitination factor E4A is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Gene ID | 9354 |
| Alternative | UBE4A; KIAA0126; Ubiquitin conjugation factor E4 A |
| Others | UBE4A Polyclonal Antibody detects endogenous levels of UBE4A protein. |
| Accession | Q14139 |
| Observed Band(KD) | 122 |
Fig.1. Western Blot analysis of extracts from Jurkat cells, using UBE4A Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.
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