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Tubulin α (Acetyl Lys40) Polyclonal Antibody

Tubulin α (Acetyl Lys40) Polyclonal Antibody

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Specification

Product name Tubulin α (Acetyl Lys40) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human Tubulin α around the acetylation site of K40
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1C; TUBA6;Tubulin alpha-6 chain; TUBA3C; TUBA2; TUBA3D; Tubulin alpha-3C/D chain; Alpha-tubulin 2; Alpha-tubulin 3C/D; Tubulin alpha-2 chain; TUBA4A; TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin;

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. TUBA1A encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that TUBA1A expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 7846
Alternative TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1C; TUBA6;Tubulin alpha-6 chain; TUBA3C; TUBA2; TUBA3D; Tubulin alpha-3C/D chain; Alpha-tubulin 2; Alpha-tubulin 3C/D; Tubulin alpha-2 chain; TUBA4A; TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin;
Others Acetyl-Tubulin α (K40) Polyclonal Antibody detects endogenous levels of Tubulin α protein only when acetylated at K40.
Accession Q71U36
Observed Band(KD) 50

Image & description

Fig.1. Western Blot analysis of Jurkat cells using Acetyl-Tubulin α (K40) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.1. Western Blot analysis of Jurkat cells using Acetyl-Tubulin α (K40) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of JK cells using Acetyl-Tubulin α (K40) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.2. Western Blot analysis of JK cells using Acetyl-Tubulin α (K40) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

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