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TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence)

Product Information

  • Product name

    TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence)

  • Application notes

    Abbkine Cell Plasma Membrane Staining Kits are a set of fluorescence imaging tools for rapid staining of plasma membranes in living and fixed suspended or attached cells depending on the cell type and experimental conditions. This kit uses a proprietary lipophilic carbocyanine dye (Ex/Em = 549/569 nm) that weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.

Fig. Hela cells stained with TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence).

Product Properties

  • Kit components

    • CPM™ Orange (1000×)
    • Assay Buffer (10×)

  • Features & Benefits

    • Enables the uniform staining of cell membrane across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells.
    • Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.
    • Proprietary CPM Orange™ (Ex/Em = 549/569 nm)-resulting in accelerated diffusion within membranes, staining is also maintained after fixation with formaldehyde, enabling further multi-color staining.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The cell membrane (plasma membrane) is a thin semi-permeable membrane, consisting of a lipid bilayer with embedded proteins that separates the interior of all cells from the environment. The basic function of the cell membrane is to protect the cell from its surroundings. The cell membrane controls the movement of substances in and out of cells and organelles. In this way, it is selectively permeable to ions and organic molecules. Cell membranes are involved in a variety of cellular processes such as cell adhesion, ion conductivity and cell signaling and serve as the attachment surface for several extracellular structures, including the cell wall, glycocalyx, and intracellular cytoskeleton.

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TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

Cat #: KTC4002

  • Size
  • Price
  • 100T $40
  • 500T $120
  • 2000T $300

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer:
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  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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