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TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence)

TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence)

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Specification

Product name TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence)
Applications notes Abbkine Cell Plasma Membrane Staining Kits are a set of fluorescence imaging tools for rapid staining of plasma membranes in living and fixed suspended or attached cells depending on the cell type and experimental conditions. The kit uses a proprietary lipophilic carbocyanine dye (Ex/Em = 484/501 nm) that weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.

Product Properties

Kit components • CPM™ Green (1000×)
• Assay Buffer (10×)
Features & Benefits • Enables the uniform staining of cell membrane across a wide variety of mammalian cell types, and can be used for both living and fixed suspended or attached cells.
• Optimized for various fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.
• Proprietary CPM Green™ (Ex/Em = 484/501 nm)-resulting in accelerated diffusion within membranes, staining is also maintained after fixation with formaldehyde, enabling further multi-color staining.
Storage instructions Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The cell membrane (plasma membrane) is a thin semi-permeable membrane, consisting of a lipid bilayer with embedded proteins that separates the interior of all cells from the environment. The basic function of the cell membrane is to protect the cell from its surroundings. The cell membrane controls the movement of substances in and out of cells and organelles. In this way, it is selectively permeable to ions and organic molecules. Cell membranes are involved in a variety of cellular processes such as cell adhesion, ion conductivity and cell signaling and serve as the attachment surface for several extracellular structures, including the cell wall, glycocalyx, and intracellular cytoskeleton.

Image & description

Fig.  Hela cells stained with TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence).

Fig. Hela cells stained with TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence).

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