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Tenascin-C Polyclonal Antibody

Tenascin-C Polyclonal Antibody

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Specification

Product name Tenascin-C Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Tenascin-C
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative TNC; HXB; Tenascin; TN; Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C; TN-C

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 240kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNC encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. Tenascin is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration.
Gene ID 3371
Alternative TNC; HXB; Tenascin; TN; Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C; TN-C
Others Tenascin-C Polyclonal Antibody detects endogenous levels of Tenascin-C protein.
Accession P24821
Observed Band(KD) 240

Image & description

Fig.1. Western Blot analysis of KB cells using Tenascin-C Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of KB cells using Tenascin-C Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

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