| Product name | Survivin Monoclonal Antibody |
| Immunogen | Recombinant Protein |
| Host | Mouse |
| Reactivity | Human,Rat |
| Applications | WB,IHC,IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000;IHC 1:200-500;IF 1:50-200 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 16kD |
| Storage buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | BIRC5 (baculoviral IAP repeat containing 5) is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but BIRC5 encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for BIRC5. |
| Gene ID | 332 |
| Others | The antibody detects endogenous Survivin protein. |
| Accession | O15392 |
Fig.1. Western blot analysis of 1) Hela, 2) 293, 3) PC12 using Survivin Monoclonal Antibody.
Fig.2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1, Survivin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue. 1, Survivin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue. 1, Survivin Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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