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Stonin-1 Polyclonal Antibody

Stonin-1 Polyclonal Antibody

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Specification

Product name Stonin-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Stonin-1 at AA rangle: 660-740
Host Rabbit
Reactivity Human
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative STON1; SALF; SBLF; STN1; Stonin-1; Stoned B-like factor

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. STON1 encodes one of two human homologs of the Drosophila melanogaster stoned B protein. Stonin-1 is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants.
Gene ID 11037
Alternative STON1; SALF; SBLF; STN1; Stonin-1; Stoned B-like factor
Others Stonin-1 Polyclonal Antibody detects endogenous levels of Stonin-1 protein.
Accession Q9Y6Q2
Observed Band(KD) 83

Image & description

Fig. Western Blot analysis of K562 cells using Stonin-1 Polyclonal Antibody.

Fig. Western Blot analysis of K562 cells using Stonin-1 Polyclonal Antibody.

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