Login Register
English
0

Cart

$ 0

Stat5 Polyclonal Antibody

Stat5 Polyclonal Antibody

Views(352) Publications(0) Catalog no(ABP52519)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Stat5 Polyclonal Antibody
Immunogen Synthesized peptide derived from human Stat5 around the non-phosphorylation site of Y694/699
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation. This phosphorylation is mediated by Src upon erythropoietin stimulation. Stat5 is constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells.
Gene ID 6776/6777
Alternative STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B
Others Stat5 Polyclonal Antibody detects endogenous levels of Stat5 protein.
Accession P42229/P51692
Observed Band(KD) 91

Image & description

Fig.1. Western Blot analysis of various cells using Stat5 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Stat5 Polyclonal Antibody.

Fig.2. Western Blot analysis of COLO205 cells using Stat5 Polyclonal Antibody.

Fig.2. Western Blot analysis of COLO205 cells using Stat5 Polyclonal Antibody.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Stat5 Polyclonal Antibody”