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STAT3 Monoclonal Antibody

STAT3 Monoclonal Antibody

Views(57) Publications(0) Catalog no(ABM40215)
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Specification

Product name STAT3 Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by STAT3 (signal transducer and activator of transcription 3) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in STAT3 are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 6774
Alternative STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Accession P40763
Observed Band(KD) 79,86

Image & description

Fig.1. Western blot analysis of 1) Rat Heart Tissue, 2) Mouse Heart Tissue, 3) Hela with STAT3 Mouse mAb diluted at 1:2000.

Fig.1. Western blot analysis of 1) Rat Heart Tissue, 2) Mouse Heart Tissue, 3) Hela with STAT3 Mouse mAb diluted at 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using STAT3Mouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using STAT3Mouse mAb diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using STAT3Mouse mAb diluted at 1:200.

Fig.3. Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using STAT3Mouse mAb diluted at 1:200.

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